| June Allyson | Jo | |
| Margaret O'Brien | Beth | |
| Janet Leigh | Meg | |
| Mary Astor | Marmee | |
| C. Aubrey Smith | Mr. Laurence | |
| Leon Ames | Mr. March |
| Peter Lawford | Laurie | |
| Elizabeth Taylor | Amy | |
| Rossano Brazzi | Professor Bhaer | |
| Lucile Watson | Aunt March | |
| Elizabeth Patterson | Hannah | |
| Harry Davenport | Dr. Barnes |