| Ben Stiller | Alex (hlas) | |
| David Schwimmer | Melman (hlas) | |
| Sacha Baron Cohen | Julien (hlas) | |
| Andy Richter | Mort (hlas) | |
| Christopher Knights | Private (hlas) | |
| Conrad Vernon | Mason (hlas) |
| Chris Rock | Marty (hlas) | |
| Jada Pinkett Smith | Gloria (hlas) | |
| Cedric the Entertainer | Maurice (hlas) | |
| Tom McGrath | Skipper/Fossa/Panicky Man on Subway (hlas) | |
| Chris Miller | Kowalski (hlas) | |
| Eric Darnell | Zoo Announcer/Lemur #1/Fossa/Subway... (hlas) |