| Ben Stiller | Alex (hlas) | |
| David Schwimmer | Melman (hlas) | |
| Sacha Baron Cohen | Julien (hlas) | |
| Andy Richter | Mort (hlas) | |
| Alec Baldwin | Makunga (hlas) | |
| Will.i.am | Moto Moto (hlas) |
| Chris Rock | Marty/Additional Zebras (hlas) | |
| Jada Pinkett Smith | Gloria (hlas) | |
| Cedric the Entertainer | Maurice (hlas) | |
| Bernie Mac | Zuba (hlas) | |
| Sherri Shepherd | Mom (hlas) | |
| Elisa Gabrielli | Nana (hlas) |